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Ty with that of the chimeras, a pattern emerges: Roc1a and ANBR promote extensive polyubiquitylation, while that of Roc1b and BNAR is lower. Similarly, Roc2 and the 2NAR chimera are both highly active in this assay (Fig. 6B). Thus, even though the RING domain is known to mediate most of the physical interaction with the E2 [11], the Roc NH2-terminus may play a significant role in determining the e

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